13-CIS RETINOYL-BETA-GLUCURONIDE IN LACRIMAL GLAND FLUID OF RABBITS TREATED WITH 13-CIS RETINOIC ACID

被引：4

作者：

UBELS, JL

OSGOOD, TB

机构：

[1] Departments of Ophthalmology and Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin

来源：

JOURNAL OF OCULAR PHARMACOLOGY | 1990年 / 6卷 / 04期

关键词：

D O I：

10.1089/jop.1990.6.321

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

When rabbits and humans are treated with orally administered 13-cis retinoic acid, and retinoic acid and a more polar retinoid metabolite appear in tears and lacrimal gland fluid. This study tested the hypothesis that this metabolite was a retinoyl-beta-glucuronide. Lacrimal gland fluid from rabbits treated with a pharmacologic dose of 13-cis retinoic acid was analyzed. Based on chromatographic retention time, absorbance maximum, and degradation to 13-cis retinoic acid by the enzyme beta-glucuronidase, it was shown that this retinoid metabolite is 13-cis retinoyl-beta-glucuronide. The 13-cis retinoyl-beta-glucuronide was also extracted from the lacrimal gland itself. it is concluded that 13-cis retinoyl-beta-glucuronide is a major metabolite of 13-cis retinoic acid in the rabbit and that this retinoid is secreted by the lacrimal gland.

引用

页码：321 / 327

页数：7

共 13 条

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