NUCLEOTIDE-SEQUENCE AND ANALYSIS OF THE VIBRIO-ALGINOLYTICUS SUCROSE UPTAKE-ENCODING REGION

被引：42

作者：

BLATCH, GL ^{[1
]}

SCHOLLE, RR ^{[1
]}

WOODS, DR ^{[1
]}

机构：

[1] UNIV CAPE TOWN,DEPT MICROBIOL,RONDEBOSCH 7700,SOUTH AFRICA

来源：

GENE | 1990年 / 95卷 / 01期

关键词：

Bacillus subtilis; fructokinase; recombinant DNA; Streptococcus mutants; sucrase; transposon;

D O I：

10.1016/0378-1119(90)90408-J

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The nucleotide sequence of the Vibrio alginolyticus sucrose uptake-encoding region was determined, and contained two genes, scrA and scrK. The scrA gene encodes an enzyme IISucrose (EIIScr) protein of the phosphoenolpyruvate dependent phosphotransferase system and the scrK gene encodes a fructokinase. The deduced amino acid (aa)_sequence for the V. alginolyticus EIIScr protein was homologous with the EIIScr protein from Streptococcus mutants, Salmonella typhimurium (pUR400 system) and Bacillus subtilis. The deduced aa sequence for the V. alginolyticus fructokinase was homologous with the Escherichia coli enzymes, 6-phosphofructokinase (isoenzyme 2) and ribokinase. Transposon phoA mutagenesis experiments indicated that the EIIScr protein was a membrane-bound protein with a region that extended into the periplasm. © 1990.

引用

页码：17 / 23

页数：7

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