OUTER-MEMBRANE CHARACTERISTICS OF SALMONELLA-ENTERITIDIS PHAGE TYPE-4 GROWING IN CHICKENS

被引：15

作者：

CHART, H ^{[1
]}

CONWAY, D ^{[1
]}

ROWE, B ^{[1
]}

机构：

[1] CENT PUBL HLTH LAB,DEPT BIOL SERV,LONDON NW9 5HT,ENGLAND

来源：

EPIDEMIOLOGY AND INFECTION | 1993年 / 111卷 / 03期

关键词：

D O I：

10.1017/S0950268800057174

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Strains of Salmonella enteritidis belonging to phage type 4 (SE4) were grown in the peritoneal cavities of chickens, and without subculture on laboratory media examined for inducible in vivo phenotypic characteristics. These bacteria expressed three major outer membrane proteins (OMPs) of 33, 35 and 36 kilodaltons (kDa), and iron regulated OMPs of 74, 78 and 81 kDa. Bacteria growing in vivo did not express flagella, or fimbriae with a subunit molecular mass of 14 kDa (14 kDa fimbriae). Two OMPs of 55 and 23 kDa, expressed during culture in nutrient broth, were repressed during growth in chickens. Possession of a 38 MDa 'mouse virulence' plasmid did not influence the expression of OMPs, flagella or fimbriae. It was concluded that strains of SE4 growing in chicken tissues, use an enterobactin mediated iron uptake system to obtain ferric ions, do not express flagella or 14 kDa fimbriae and appear not to express novel OMPs involved in survival in vivo.

引用

页码：449 / 454

页数：6

共 16 条

[1] Threlfall E.J., Rowe B., Ward L.R., Subdivision of Salmonella enteritidis phage types by plasmid typing, Epidemiol Infect, 102, (1989)
[2] Coyle E.F., Palmer S.R., Riberio C.D., Salmonella enteritidis phage type 4 infection: Association with hens’ eggs, Lancet, ii, (1988)
[3] Smith J.G.W., Memorandum of evidence to the agricultural committee enquiry on salmonella in eggs, PHLS Microbiol Digest, 6, pp. 1-9, (1989)
[4] Sharp J.C.M., Salmonellosis and eggs, BMJ, 297, (1988)
[5] Rampling A., Anderson J.R., Upson R., Peters E., Ward L.R., Rowe B., Salmonella enteritidis phage type 4 infection of chickens: A hazard to public health, Lancet, ii, (1989)
[6] Humphrey T.J., Whitehead A., Gawler A.H.L., Henley A., Rowe B., Numbers of Salmonella enteritidis in the contents of naturally contaminated hens’ eggs, Epidemiol Infect, 106, (1991)
[7] Humphrey T.J., Baskerville A., Mawer S., Rowe B., Hopper S., Salmonela enteritidis phage type 4 from the contents of intact eggs: a study involving naturally infected hens, Epidemiol Infect, 103, (1989)
[8] Feutrier J., Kay W.W., Trust T.K., Purification and characterization of fimbriae from Salmonella enteritidis, J Bacteriol, 168, (1986)
[9] Muller K-H., Collinson K., Trust T.J., Kay W.W., Type 1 fimbriae of Salmonella enteritidis, J Bacteriol, 173, (1991)
[10] Collinson S.K., Emody L., Muller K-H., Trust T.J., Kay W.W., Purification and characterization of thin, aggregative fimbriae from Salmonella enteritidis, J Bacteriol, 173, (1991)

← 1 2 →