Similar binding properties for a neutralizing anti-tetanus toxoid human monoclonal antibody and its bacterially expressed Fab

被引：16

作者：

Lafaye, P ^{[1
]}

Nato, F ^{[1
]}

Mazie, JC ^{[1
]}

Doyen, N ^{[1
]}

机构：

[1] INST PASTEUR, HYBRIDOLAB, F-75724 PARIS 15, FRANCE

来源：

RESEARCH IN IMMUNOLOGY | 1995年 / 146卷 / 06期

关键词：

mAb; affinity; Fab; human mAb anti-tetanus antibody; phage display expression system; neutralizing activity;

D O I：

10.1016/0923-2494(96)81041-8

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A high-affinity anti-tenanus toroid (TT) human monoclonal antibody showing neutralizing activity was isolated from a fusion between mouse myeloma and human splenic cells. Fab fragments from this antibody were obtained using a recombinant phage surface-display expression system. The parental antibody and the corresponding Fab had identical immunological activities, including specificity and affinity. These results confirm the feasibility of developing Escherichia coli expression of monoclonal human Fab from hybridoma cells.

引用

收藏

页码：373 / 382

页数：10

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