PURIFICATION OF ANTIGENIZED IMMUNOGLOBULINS DERIVATIZED WITH MONOMETHOXYPOLYETHYLENE GLYCOL

被引：14

作者：

BRUMEANU, TD ^{[1
]}

ZAGHOUANI, H ^{[1
]}

BONA, C ^{[1
]}

机构：

[1] MT SINAI SCH MED,DEPT MICROBIOL,NEW YORK,NY 10029

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 696卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)01275-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetically engineered immunoglobulins (Igs) carrying viral B or T cell peptides in the CDR3 loop, function as efficient delivery system of the defined viral epitopes. Two of these antigenized Igs (AIgs) were derivatized with 2-O-monomethoxypolyethylene glycol-4,6-dichloro-s-triazine (mPEG). Herein, we describe a two-step strategy to purify mPEG-derivatized AIgs (AIgs-mPEG). Unreacted mPEG polymers were removed by Size-exclusion chromatography using ammonium hydrogencarbonate as a buffer system. Mildly PEGylated AIgs were isolated from free and highly derivatized AIgs by anion-exchange chromatography. Electrophoretic analysis indicated that the AIgs-mPEG preparation contained less than 4 . 10(-4) M unreacted mPEG. This strategy may be applied to other mPEG-derivatized monoclonal antibodies.

引用

页码：219 / 225

页数：7

共 26 条

[1]

ABUCHOWSKI A, 1977, J BIOL CHEM, V252, P3582

[2]

ABUCHOWSKI A, 1977, J BIOL CHEM, V252, P3578

[3] EPITOPE-SPECIFIC SUPPRESSION OF ANTIBODY-RESPONSE IN EXPERIMENTAL AUTOIMMUNE MYASTHENIA-GRAVIS BY A MONOMETHOXYPOLYETHYLENE GLYCOL CONJUGATE OF A MYASTHENOGENIC SYNTHETIC PEPTIDE [J].