MICROTUBULE-ASSOCIATED PROTEIN 2(MAP-2) - A SENSITIVE MARKER OF SEIZURE-RELATED BRAIN-DAMAGE

被引：50

作者：

BALLOUGH, GPH

MARTIN, LJ

CANN, FJ

GRAHAM, JS

SMITH, CD

KLING, CE

FORSTER, JS

PHANN, S

FILBERT, MG

机构：

[1] USA,MED RES INST CHEM DEF,DIV PATHOPHYSIOL,NEUROTOXICOL BRANCH,ABERDEEN PROVING GROUND,MD 21010

[2] JOHNS HOPKINS UNIV,SCH MED,DEPT PATHOL,BALTIMORE,MD 21205

[3] JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROSCI,BALTIMORE,MD 21205

[4] LA SALLE UNIV,DEPT BIOL,PHILADELPHIA,PA 19141

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1995年 / 61卷 / 1-2期

关键词：

MICROTUBULE-ASSOCIATED PROTEIN-2; SEIZURE; NEUROTOXICITY; SOMAN; GFAP; CRESYL VIOLET; HEMATOXYLIN; EOSIN;

D O I：

10.1016/0165-0270(95)00019-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have assessed the efficacy of MAP-2 immunohistochemistry as a marker of seizure-related brain damage and its suitability for quantitation of the damage using densitometric and morphometric image analysis. Seizures were produced in rats by administration of 1.5 LD(50) soman, an irreversible AChE inhibitor. Our results demonstrate that neuronal damage, assessed using hematoxylin and eosin, and cresyl violet staining, was colocalized on adjacent serial sections with clearly demarcated reductions in MAP-2 staining. The most severely damaged brain regions were devoid of MAP-2 staining. Reductions in MAP-2 immunostaining were found to be exceptionally well suited for quantitation using densitometric and morphometric image analysis. This study represents the first demonstration of seizure-induced excitotoxic alterations in MAP-2.

引用

页码：23 / 32

页数：10

共 32 条

[1] LIGHT AND ELECTRON-MICROSCOPIC STUDIES OF THE DISTRIBUTION OF MICROTUBULE-ASSOCIATED PROTEIN-2 IN RAT-BRAIN - A DIFFERENCE BETWEEN DENDRITIC AND AXONAL CYTOSKELETONS [J].