SEQUENCE-ANALYSIS OF THE PROMOTER REGION OF THE GLIOBLASTOMA DERIVED T-CELL SUPPRESSOR FACTOR TRANSFORMING GROWTH-FACTOR (TGF)-BETA-2 GENE REVEALS STRIKING DIFFERENCES TO THE TGF-BETA-1 AND TGF-BETA-3 GENES

Human glioblastoma cells secrete a factor termed glioblastoma derived T cell suppressor factor (G-TsF) or transforming growth factor β2 (TGF-β2 which inhibits the response of T cells to mitogenic or antigenic stimulation. In the present study we isolated the promoter region of the G-TsF TGF-β2 gene. The promoter region shares no homology to the promoter of the TGF-β1 or the 5′ region of the TGF-β3 gene and harbours several familiar DNA motifs, including the cytokine-1 region, an octamer-like sequence, Sp1- and AP-2-like elements and a putative NF-κb site. In contrast to the TGF-β1 gene, the G-TsF TGF-β2 gene contains three TATA-like sequences but lacks an AP-1 site. To understand the cell type specificity of expression of G-TsF TGF-β2, the individual contribution of the DNA elements detected in the promoter has to be analysed in further studies. © 1990.