ALPHA-ACTININ ACCUMULATION IN EPITHELIAL-CELLS INFECTED WITH ATTACHING AND EFFACING GASTROINTESTINAL PATHOGENS

被引：21

作者：

ISMAILI, A

PHILPOTT, DJ

DYTOC, MT

SONI, R

RATNAM, S

SHERMAN, PM

机构：

[1] HOSP SICK CHILDREN,RES INST,DIV GASTROENTEROL,TORONTO,ON M5G 1X8,CANADA

[2] UNIV TORONTO,DEPT MICROBIOL,TORONTO,ON M5S 1A1,CANADA

[3] UNIV TORONTO,DEPT PEDIAT,TORONTO,ON,CANADA

[4] NEWFOUNDLAND & LABRADOR PUBL HLTH LABS,ST JOHNS,NF,CANADA

来源：

JOURNAL OF INFECTIOUS DISEASES | 1995年 / 172卷 / 05期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1093/infdis/172.5.1393

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Fluorescent F-actin staining utilizing phalloidin, a highly toxic mushroom poison, is used as an indirect test to detect attaching and effacing (AE) bacteria. A study was done to determine if accumulation of alpha-actinin in infected tissue culture cells is a consistent feature and whether it corresponds with the AE response. Rearrangement of alpha-actinin was detected using immunofluorescence microscopy by incubation of infected cells with a murine monoclonal anti-alpha-actinin antibody, Foci of alpha-actinin-specific fluorescence corresponding to areas of bacterial adhesion were detected by transmission electron microscopy in HEp-2 and gastric KATO-III cells infected with only those bacterial strains that formed AE lesions. Therefore, this study shows that alpha-actinin accumulation is a consistent, specific manifestation of the AE phenotype and forms the basis for the development of a safe alternative test for detecting AE bacteria.

引用

页码：1393 / 1396

页数：4

共 15 条

[1] NON-MUSCLE ALPHA-ACTININS ARE CALCIUM-SENSITIVE ACTIN-BINDING PROTEINS [J].