AN IMPROVED METHOD FOR THE PREPARATION OF RAT CEREBELLAR GLOMERULI

被引：6

作者：

VIENNOT, F ^{[1
]}

ARTAULT, JC ^{[1
]}

THOLEY, G ^{[1
]}

DEBARRY, J ^{[1
]}

GOMBOS, G ^{[1
]}

机构：

[1] INSERM, U44, F-67084 STRASBOURG, FRANCE

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1991年 / 38卷 / 01期

关键词：

GLOMERULI; CEREBELLUM; CHOLINE; AAT; GDH;

D O I：

10.1016/0165-0270(91)90154-R

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cerebellar glomeruli consist of large portions of the mossy fiber giant terminal, granule cell dendrites and Golgi neuron terminals. By modifying previously reported procedures we have developed a new method for bulk preparation of this polysynaptic complex from rat cerebellum. We obtained well preserved isolated glomeruli of satisfactory purity and homogeneity as indicated by electron microscopy and by determination of appropriate biochemical markers. The method is fast and simple, and it provides a glomerular fraction suitable for investigation of neurotransmitter receptors.

引用

页码：51 / 62

页数：12

共 37 条

[1] SUBCELLULAR FRACTIONATION OF RAT CEREBELLUM - ELECTRON-MICROSCOPIC AND BIOCHEMICAL INVESTIGATION .3. ISOLATION OF LARGE FRAGMENTS OF CEREBELLAR GLOMERULI [J].