RAINBOW-TROUT P53 - CDNA CLONING AND BIOCHEMICAL-CHARACTERIZATION

被引：67

作者：

DEFROMENTEL, CC

PAKDEL, F

CHAPUS, A

BANEY, C

MAY, P

SOUSSI, T

机构：

[1] INST RECH SCI CANC,UNITE ONCOL MOLEC,F-94802 VILLEJUIF,FRANCE

[2] FAC SCI RENNES,BIOL MOLEC LAB,CNRS,URA 256,F-35042 RENNES,FRANCE

[3] ECOLE NATL CHIM,F-75013 PARIS,FRANCE

来源：

GENE | 1992年 / 112卷 / 02期

关键词：

RECOMBINANT DNA; FISH; TUMOR-SUPPRESSOR GENE; DNA SEQUENCING; EVOLUTIONARY CONSERVATION; SV40 LARGE T-ANTIGEN;

D O I：

10.1016/0378-1119(92)90383-Z

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have cloned and sequenced the p53-encoding cDNA of rainbow trout (Salmo gairdneri). The encoded product contains the characteristics found in all p53 proteins: (i) the five highly conserved domains, (ii) an acidic N terminus, (iii) a hydrophilic C terminus, and (iv) a penultimate serine residue. Furthermore, we demonstrate that the rainbow trout p53 is able to specifically interact with the SV40 large T antigen.

引用

页码：241 / 245

页数：5

共 14 条

[1] UNIDIRECTIONAL DIGESTION WITH EXONUCLEASE-III CREATES TARGETED BREAKPOINTS FOR DNA SEQUENCING [J].