DETERMINATION OF INORGANIC-PHOSPHATE IN DRUG FORMULATIONS AND BIOLOGICAL-FLUIDS USING A PLANT-TISSUE ELECTRODE

被引：16

作者：

CAMPANELLA, L ^{[1
]}

CORDATORE, M ^{[1
]}

MAZZEI, F ^{[1
]}

TOMASSETTI, M ^{[1
]}

机构：

[1] NATL COUNCIL RES, SSPS, ROME, ITALY

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1990年 / 8卷 / 8-12期

关键词：

PHOSPHATE; ANALYSIS; TISSUE-ENZYME HYBRID BIOSENSOR; DRUGS; BIOLOGICAL FLUIDS;

D O I：

10.1016/0731-7085(90)80108-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A suitable biosensor for the analysis of inorganic phosphate was developed by coupling the enzyme glucose oxidase to a slice of potato (Solanum tuberosum) containing the acid phosphatase, and using an amperometric Clark electrode as an indicating sensor. Phosphate concentration can be determined from its inhibition on the acid phosphatase activity. Measurements were carried out at steady-state conditions and the available concentration range of phosphate, repeatability and accuracy, on phosphate standard solutions, were experimentally determined. Finally the sensor was used in pharmaceutical and biological matrices. The results were compared with those ones obtained by Bartlett's spectrometric method.

引用

页码：711 / 716

页数：6

共 6 条

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[3] Fiske CH, 1925, J BIOL CHEM, V66, P375
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[5] HYBRID ELECTRODE FOR THE DETERMINATION OF PHOSPHATES AND POLYPHOSPHATES - APPLICATION TO ENVIRONMENTAL-PROBLEMS
LINDERS, CR
VINCKE, BJ
PATRIARCHE, GJ
[J]. ANALYTICAL LETTERS PART B-CLINICAL AND BIOCHEMICAL ANALYSIS, 1985, 18 (17): : 2195 - 2208
[6] PLANT-TISSUE HYDRID ELECTRODE FOR DETERMINATION OF PHOSPHATE AND FLUORIDE
SCHUBERT, F
RENNEBERG, R
SCHELLER, FW
KIRSTEIN, L
[J]. ANALYTICAL CHEMISTRY, 1984, 56 (09) : 1677 - 1682

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