IDENTIFICATION OF ANTIBODY EPITOPES WITHIN THE CB-11 PEPTIDE OF TYPE-II COLLAGEN .1. DETECTION OF ANTIBODY-BINDING SITES BY EPITOPE SCANNING

被引：13

作者：

WORTHINGTON, J ^{[1
]}

BRASS, A ^{[1
]}

MORGAN, K ^{[1
]}

机构：

[1] UNIV MANCHESTER,SCH MED,DEPT RHEUMATOL,STOPFORD BLDG,OXFORD RD,MANCHESTER M13 9PT,LANCS,ENGLAND

来源：

AUTOIMMUNITY | 1991年 / 10卷 / 03期

关键词：

TYPE-II COLLAGEN; CB-11; PEPTIDE; ANTIBODIES; EPITOPE SCANNING; ARTHRITIS; ANIMAL MODEL;

D O I：

10.3109/08916939109001890

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Using epitope scanning, the precise location of antibody binding sites on the CB-11 peptide of bovine type II collagen have been identified for the first time. Two hundred and seventy two peptides (8 amino acids in length and overlapping by seven amino acids), representing the complete CB-11 sequence, were synthesised on solid phase supports, in duplicate, and were screened with sera from arthritic and non-arthritic, bovine type II collagen-immunised rats. A total of twenty one different antibody binding sites were identified with no epitope being uniquely recognised by sera from arthritic, as compared to non-arthritic, rats although differences in the relative amount of antibody binding were seen. Individual sera identified between two and thirteen epitopes with one epitope being recognised by all sera. Some of the amino acid sequences, of the CB-11 region of bovine type II collagen, recognised by the rat sera are identical to the sequences in human type II collagen and thus these epitopes may be relevant to auto-immunity to type II collagen in patients with rheumatoid arthritis. © 1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：201 / 207

页数：7

共 25 条

[1]

Andriopoulos N.A., Mestecky Miller J.E.J., Antibodies to native and denatured collagens in sera of patients with rheumatoid arthritis, Arthritis Rheum, 19, pp. 613-617, (1976)

[2]

Clague R.B., Shaw M.J., Holt P.J.L., Incidence and correlation between serum IgG and IgM antibodies to native type II collagen in patients with chronic inflammatory arthritis, Ann Rheum Dis, 40, pp. 6-10, (1981)

[3]

Clague R.B., Firth S.A., Holt P.J.L., Skingle J., Greenbury C.L., Webley M., Serum antibodies to type II collagen in rheumatoid arthritis: comparison of six immunological methods and clinical features, Ann Rheum Dis, 42, pp. 537-544, (1983)

[4]

Trentham D.E., Kammer G.M., Mc Cune W.J., David J.R., Autoimmunity to collagen: a shared feature of psoriatic and rheumatoid arthritis, Arthritis Rheum, 24, pp. 1363-1369

[5]

Klimiuk P.S., Clague R.B., Grennan D.M., Dyer P.A., Smcaton Harris I.R., Autoimmunity to native type II collagen-a distinct genetic subset of rheumatoid arthritis, J Rheumatol, 12, pp. 865-870, (1985)

[6]

Choi E.K., Gatenby P.A., Me Gill N.W., Bateman J.F., Cole W.G., York J.R., Autoantibodies to type II collagen: occurrence in rheumatoid arthritis, other arthritides. autoimmune connective tissue diseases and chronic inflammatory syndromes, Ann Rheum Dis, 47, pp. 313-322, (1988)

[7]

Trentham D.E., Townes A.S., Kang A.H., Autoimmunity to type II collagen: an experimental model of arthritis, J Exp Med, 146, pp. 857-867, (1977)

[8]

Morgan K., Clague R.B., Shaw M.J., Holt R.J.L., Native type 11 collagen-induced arthritis in the rat. I. Incidence and humoral response to collagen, Ann Rheum Dis, 39, pp. 285-290, (1980)

[9]

Stuart J.M., Cremer M.A., Kang A.H., Townes A.S., Collagen induced arthritis in rats: evaluation of early immunological events, Arthritis Rheum, 22, pp. 1344-1351, (1979)

[10]

Staines N.A., Hardingham Smith T., Henderson M.B., Collagen-induced arthritis in the rat: modification of immune and arthritic responses by free collagen and immune anti-collagen antiserum, Immunology, 44, pp. 737-744, (1981)

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