SUBUNIT-DEPENDENT ASSEMBLY OF INWARD-RECTIFIER K+ CHANNELS

被引：109

作者：

GLOWATZKI, E ^{[1
]}

FAKLER, G ^{[1
]}

BRANDLE, U ^{[1
]}

REXHAUSEN, U ^{[1
]}

ZENNER, HP ^{[1
]}

RUPPERSBERG, JP ^{[1
]}

FAKLER, B ^{[1
]}

机构：

[1] UNIV TUBINGEN, ENT HOSP, DEPT SENSORY BIOPHYS, D-72076 TUBINGEN, GERMANY

来源：

PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES | 1995年 / 261卷 / 1361期

关键词：

D O I：

10.1098/rspb.1995.0145

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Inward-rectifier, G-protein-regulated and ATP-dependent K+ channels form a novel gene family of related proteins which share two transmembrane segments as a common structural feature. These K+ channels are only distantly related to the voltage-gated Shaker-type K+ channels comprising six transmembrane segments. Although the quaternary structure of voltage-gated K+ channels has been extensively studied in the past, little is known about subunit assembly of inward-rectifier K+ channels. Differential sensitivity of inward-rectifier K+ channels to voltage-dependent pore block by spermine was used to analyse subunit assembly. It is shown that inward-rectifier K+ channel proteins are composed of four subunits whose assembly obeys the rules of a binomial distribution. 'Strong' and 'mild' inward-rectifier K+ channel subunits (BIR10 and ROMK1) which are co-expressed in individual auditory hair cells form hetero-tetramers. Distribution of these hetero-tetramers, however, is not binomial. Hetero- and homo-oligomeric channels form with similar probabilities resulting in independent channel populations with distinct functional properties.

引用

页码：251 / 261

页数：11

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