THE SCHIFF-BASE BETWEEN PYRIDOXAL-5'-PHOSPHATE (PLP) AND HEXYLAMINE - FORMATION OF THE UNPROTONATED FORM OF THE IMINE BY REACTION OF THE UNPROTONATED PLP AND FREE AMINE

被引：4

作者：

BLAZQUEZ, M

PINEDA, T

SEVILLA, JM

DOMINGUEZ, M

GARCIABLANCO, F

机构：

[1] UNIV SEVILLA,DEPT QUIM FIS,E-41072 SEVILLE,SPAIN

[2] UNIV COMPLUTENSE MADRID,FAC FARM,DEPT FIS QUIM,E-28032 MADRID,SPAIN

来源：

JOURNAL OF PHYSICAL ORGANIC CHEMISTRY | 1991年 / 4卷 / 06期

关键词：

D O I：

10.1002/poc.610040609

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Electrochemical and spectrophotometric studies of the pyridoxal-5'-phosphate hexylamine Schiff base (PHSB) in strongly basic media were carried out. The equilibrium constant and rate constants of the formation and hydrolysis of the unprotonated imine from the unprotonated pyridoxal-5'-phosphate and free amine were determined by linear-scan cyclic voltammetry. The acid-base dissociation constant of the imine group was obtained by spectrophotometric measurements. Absorption bands for the free imine and the conjugate acid are characterized by a log-normal distribution. These studies are for general application to the quantitative characterization of non-enzymatic model compounds.

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页码：372 / 380

页数：9

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