SHORT-TERM EFFECT OF ALDOSTERONE ON VASOPRESSIN-SENSITIVE ADENYLATE-CYCLASE IN RAT COLLECTING TUBULE

被引:11
作者
ELMERNISSI, G
BARLETBAS, C
KHADOURI, C
CHEVAL, L
MARSY, S
DOUCET, A
机构
[1] COLL FRANCE,PHYSIOL CELLULAIRE LAB,CNRS,UNITE RECH ASSOCIEE 219,11 PL M BERTHELOT,F-75231 PARIS 05,FRANCE
[2] UNIV CADI AYYAD,FAC SCI,BIOMEMBRANES LAB,MARRAKECH,MOROCCO
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1993年 / 264卷 / 05期
关键词
CORTICAL COLLECTING TUBULE; MEDULLARY COLLECTING TUBULE; ADRENALECTOMY; SPIRONOLACTONE; RU-38486;
D O I
10.1152/ajprenal.1993.264.5.F821
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Because previous studies indicated that, in the rat collecting tubule, vasopressin (AVP)-sensitive adenylate cyclase (AC) is controlled by mineralocorticoids in the long term, the present study was designed to investigate whether such a control also exists in the short term. Therefore, we investigated the in vivo and in vitro effects of aldosterone on AC activity in cortical and outer medullary collecting tubules (CCD and OMCD, respectively) from adrenalectomized rats. Injection of aldosterone (10 mug/kg body wt) to adrenalectomized rats restored within 3 h AVP-sensitive AC activity in the CCD and OMCD up to the levels observed in the corresponding segments of adrenal intact rats. Similarly, incubating CCD or OMCD from adrenalectomized rats for 2.5 h in the presence of 10(-8) M aldosterone enhanced AVP-sensitive AC activity up to values similar to those found in normal rats. In vitro stimulation of AVP-sensitive AC activity was dose dependent with regard to aldosterone [apparent affinity constant (K0.5) almost-equal-to 10(-9) M], appeared after a 30-min lag period, and reached its maximum after 2-2.5 h. In addition, it was totally abolished by the antiminer-alocorticoid spironolactone, whereas the specific glucocorticoid antagonist RU 38486 had no effect. Finally, actinomycin D and cycloheximide totally abolished the in vitro action of aldosterone, demonstrating the involvement of protein synthesis in that process.
引用
收藏
页码:F821 / F826
页数:6
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