THE DESIGN OF ENZYME SENSORS BASED ON THE ENZYME STRUCTURE

被引：47

作者：

ALVAREZICAZA, M ^{[1
]}

KALISZ, HM ^{[1
]}

HECHT, HJ ^{[1
]}

AUMANN, KD ^{[1
]}

SCHOMBURG, D ^{[1
]}

SCHMID, RD ^{[1
]}

机构：

[1] GESELL BIOTECHNOL FORSCH MBH,D-38124 BRAUNSCHWEIG,GERMANY

来源：

BIOSENSORS & BIOELECTRONICS | 1995年 / 10卷 / 08期

关键词：

GLUCOSE OXIDASE; DIRECT ELECTRON TRANSFER; ELECTRON TUNNELING; ELECTRON PATHWAY; ORIENTED ENZYME IMMOBILIZATION;

D O I：

10.1016/0956-5663(95)96964-Z

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The size of some of the reported electron mediators for glucose oxidase compared with the available space to penetrate the active site, implies that electrons have to move along the protein structure. Theoretical and experimental evidence predicts that it is possible to have electron transfer at the required rate used in biosensors (200 to 1840 electrons s(-1)) for the distances in glucose oxidase (20 to 25 Angstrom). Use of the program ''Pathways'' (together with the knowledge of the enzyme structure) allowed us to find an electron pathway within the enzyme. This pathway has a maximum electron coupling between the active site and the surface of the enzyme. The pathway reaches the surface near functional groups that can be used for oriented immobilization of the enzyme. Experimental confirmation of this particular pathway has been attempted but it is still elusive.

引用

页码：735 / 742

页数：8

共 20 条

[1] OBSERVATION OF DIRECT ELECTRON-TRANSFER FROM THE ACTIVE-CENTER OF GLUCOSE-OXIDASE TO A GRAPHITE ELECTRODE ACHIEVED THROUGH THE USE OF MILD IMMOBILIZATION [J].

ALVAREZICAZA, M ;

SCHMID, RD .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1994, 33 (02) :191-199

[2] MAPPING ELECTRON-TUNNELING PATHWAYS - AN ALGORITHM THAT FINDS THE MINIMUM LENGTH MAXIMUM COUPLING PATHWAY BETWEEN ELECTRON-DONORS AND ACCEPTORS IN PROTEINS [J].

BETTS, JN ;

BERATAN, DN ;

ONUCHIC, JN .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (11) :4043-4046

[3] FERROCENE-MEDIATED ENZYME ELECTRODE FOR AMPEROMETRIC DETERMINATION OF GLUCOSE [J].

CASS, AEG ;

DAVIS, G ;

FRANCIS, GD ;

HILL, HAO ;

ASTON, WJ ;

HIGGINS, IJ ;

PLOTKIN, EV ;

SCOTT, LDL ;

TURNER, APF .

ANALYTICAL CHEMISTRY, 1984, 56 (04) :667-671

[4] DIRECT ELECTRICAL COMMUNICATION BETWEEN CHEMICALLY MODIFIED ENZYMES AND METAL-ELECTRODES .1. ELECTRON-TRANSFER FROM GLUCOSE-OXIDASE TO METAL-ELECTRODES VIA ELECTRON RELAYS, BOUND COVALENTLY TO THE ENZYME [J].

DEGANI, Y ;

HELLER, A .

JOURNAL OF PHYSICAL CHEMISTRY, 1987, 91 (06) :1285-1289

[5] STUDIES OF PHOTOSYNTHESIS USING A PULSED LASER .I. TEMPERATURE DEPENDENCE OF CYTOCHROME OXIDATION RATE IN CHROMATIUM . EVIDENCE FOR TUNNELING [J].

DEVAULT, D ;

CHANCE, B .

BIOPHYSICAL JOURNAL, 1966, 6 (06) :825-&

[6] EFFECTIVE COUPLING IN BIOLOGICAL ELECTRON-TRANSFER - EXPONENTIAL OR COMPLEX DISTANCE DEPENDENCE [J].

EVENSON, JW ;

KARPLUS, M .

SCIENCE, 1993, 262 (5137) :1247-1249

[7] CROSS-LINKED REDOX GELS CONTAINING GLUCOSE-OXIDASE FOR AMPEROMETRIC BIOSENSOR APPLICATIONS [J].

GREGG, BA ;

HELLER, A .

ANALYTICAL CHEMISTRY, 1990, 62 (03) :258-263

[8] A NEW CLASS OF AMPEROMETRIC BIOSENSOR INCORPORATING A POLYMERIC ELECTRON-TRANSFER MEDIATOR [J].

HALE, PD ;

INAGAKI, T ;

KARAN, HI ;

OKAMOTO, Y ;

SKOTHEIM, TA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (09) :3482-3484

[9] COVALENTLY ATTACHED ORGANIC MONOLAYERS ON SEMICONDUCTOR SURFACES [J].

HALLER, I .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1978, 100 (26) :8050-8055

[10] CRYSTAL-STRUCTURE OF GLUCOSE-OXIDASE FROM ASPERGILLUS-NIGER REFINED AT 2 .3 ANGSTROM RESOLUTION [J].

HECHT, HJ ;

KALISZ, HM ;

HENDLE, J ;

SCHMID, RD ;

SCHOMBURG, D .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 229 (01) :153-172

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