A SINGLE EXPRESSION SYSTEM FOR THE DISPLAY, PURIFICATION AND CONJUGATION OF SINGLE-CHAIN ANTIBODIES

被引：8

作者：

HAYASHI, N ^{[1
]}

KIPRIYANOV, S ^{[1
]}

FUCHS, P ^{[1
]}

WELSCHOF, M ^{[1
]}

DORSAM, H ^{[1
]}

LITTLE, M ^{[1
]}

机构：

[1] GERMAN CANC RES CTR,RECOMBINANT ANTIBODY GRP,D-69120 HEIDELBERG,GERMANY

来源：

GENE | 1995年 / 160卷 / 01期

关键词：

PHAGE DISPLAY; RECOMBINANT ANTIBODY; SINGLE-CHAIN (FV) ANTIBODY; IMMUNOGLOBULIN; ESCHERICHIA COLI; PHAGEMID; ANTIBODY LIBRARY; LIBRARY SCREENING;

D O I：

10.1016/0378-1119(95)00218-U

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

To facilitate the purification and conjugation of single-chain antibodies (scFv) selected from a phage display library, we have incorporated His(6), an amber stop codon and a C-terminal Cys into a surface expression vector. The vector also contains a lacl(q) gene for improving the efficiency of regulation and a sequence coding for a marker peptide.

引用

页码：129 / 130

页数：2

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