REQUIREMENT FOR GENERATION OF H2O2 FOR PLATELET-DERIVED GROWTH-FACTOR SIGNAL-TRANSDUCTION

被引：2255

作者：

SUNDARESAN, M

YU, ZX

FERRANS, VJ

IRANI, K

FINKEL, T

机构：

[1] NHLBI, CARDIOL BRANCH, BETHESDA, MD 20892 USA

[2] NHLBI, PATHOL SECT, BETHESDA, MD 20892 USA

来源：

SCIENCE | 1995年 / 270卷 / 5234期

关键词：

D O I：

10.1126/science.270.5234.296

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Stimulation of rat vascular smooth muscle cells (VSMCs) by platelet-derived growth factor (PDGF) transiently increased the intracellular concentration of hydrogen peroxide (H2O2). This increase could be blunted by increasing the intracellular concentration of the scavenging enzyme catalase or by the chemical antioxidant N-acetylcysteine. The response of VSMCs to PDGF, which includes tyrosine phosphorylation, mitogen-activated protein kinase stimulation, DNA synthesis, and chemotaxis, was inhibited when the growth factor-stimulated rise in H2O2 concentration was blocked. These results suggest that H2O2 may act as a signal-transducing molecule, and they suggest a potential mechanism for the cardioprotective effects of antioxidants.

引用

页码：296 / 299

页数：4

共 31 条

[1] DIFFERENTIAL-EFFECTS OF PLATELET-DERIVED GROWTH-FACTOR BB ON P125 FOCAL ADHESION KINASE AND PAXILLIN TYROSINE PHOSPHORYLATION AND ON CELL-MIGRATION IN RABBIT AORTIC VASCULAR SMOOTH-MUSCLE CELLS AND SWISS 3T3 FIBROBLASTS [J].