ECDYSTEROIDS IN RELATION TO THE MOLT CYCLE OF THE AMERICAN LOBSTER, HOMARUS-AMERICANUS .2. EXCRETION OF METABOLITES

Ecdysteroid (Ecd) excretion patterns were followed during the molt cycle of adult male and female lobsters, Homarus americanus. Urine was the major route of Ecd elimination, amounting to ≥96% of the excreted radioimmunoassay activity for all molt stages. The other identified route of Ecd elimination from the hemolymph was the feces, which accounted for the remaining 4% of the total Ecd excretion. High polarity metabolites (HP), including 20,26-dihydroxyecdysone (2026E) and 20-hydroxyecdysonoic acid (20EA), were the major types of Ecds found in the urine. Other urinary Ecd components included 20-hydroxyecdysone (20E), ecdysone (E), and ponasterone A (P). The major portion of urinary HP was composed of conjugates of 2026E, 20E, E, P, and other unidentified metabolites. The fecal Ecds were predominately HP and apolar metabolites. Apolar fecal Ecds were hydrolyzable to release 20EA, 2026E, 20E, E, P, and other metabolites. By means of intubation, [3H]E was placed directly into the cardiac stomach of lobsters. The gut pathway formed an apolar conjugate of [3H]E which was found exclusively in the feces. Lobsters are therefore capable of excreting ingested Ecds without absorption. © 1991.