MOLECULAR CYTOGENETICS OF ALPHA-SATELLITE DNA FROM CHROMOSOME-12 - FLUORESCENCE INSITU HYBRIDIZATION AND DESCRIPTION OF DNA AND ARRAY LENGTH POLYMORPHISMS

被引：23

作者：

GREIG, GM

PARIKH, S

GEORGE, J

POWERS, VE

WILLARD, HF

机构：

[1] STANFORD UNIV,MED CTR,SCH MED,DEPT GENET,STANFORD,CA 94305

[2] UNIV TORONTO,DEPT MED GENET,TORONTO M5S 1A1,ONTARIO,CANADA

[3] ONCOR INC,DIV MOLEC CYTOGENET,GAITHERSBURG,MD

来源：

CYTOGENETICS AND CELL GENETICS | 1991年 / 56卷 / 3-4期

关键词：

D O I：

10.1159/000133071

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A 340-bp EcoRI fragment of alpha satellite DNA from human chromosome 12 has been isolated and used in molecular cytogenetic and genetic studies. The clone, pSP12-1, detects tandemly repeated 1.4-kb repeat units at the centromeric region of chromosome 12. By fluorescence in situ hybridization, biotinylated pSP12-1 is highly specific for chromosome 12 and has been used to confirm an i(12p) in a case of Pallister-Killian syndrome, both in metaphase spreads and in interphase nuclei. A dominant DNA polymorphism for the centromeric D12Z3 locus is detected with the enzyme TaqI. In addition, a high frequency of D12Z3 array length polymorphisms can be detected using pulsed-field gel electrophoresis. The D12Z3 array has been measured by pulsed-field gel electrophoresis to span approximately 2,250-4,300 kb at the centromeric region of chromosome 12.

引用

页码：144 / 148

页数：5

共 37 条

[1] THE PHYLOGENY OF HUMAN-CHROMOSOME SPECIFIC ALPHA-SATELLITES [J].