EXPRESSION OF A BACTERIAL PHASEOLOTOXIN-RESISTANT ORNITHYL TRANSCARBAMYLASE IN TRANSGENIC TOBACCO CONFERS RESISTANCE TO PSEUDOMONAS-SYRINGAE PV PHASEOLICOLA

被引：26

作者：

DELAFUENTEMARTINEZ, JM ^{[1
]}

MOSQUEDACANO, G ^{[1
]}

ALVAREZMORALES, A ^{[1
]}

HERRERAESTRELLA, L ^{[1
]}

机构：

[1] CTR INVEST & ESTUDIOS AVANZADOS,DEPT INGN GENET PLANTAS,APARTADO POSTAL 629,IRAPUATO 3600,GUANAJUATO,MEXICO

来源：

BIO-TECHNOLOGY | 1992年 / 10卷 / 08期

关键词：

D O I：

10.1038/nbt0892-905

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Toxins have been shown to be an important virulence component for most pathovars of Pseudomonas syringae. Here we have examined the role of phaseolotoxin in the virulence mechanism of P. syringae pv. phaseolicola by producing transgenic tobacco plants that express a pathogen-derived toxin-resistant target enzyme. Such plants are insensitive to the toxin and less prone to infection by the pathogen.

引用

页码：905 / 909

页数：5

共 30 条

[1] TRANSGENIC TOBACCO RESISTANT TO A BACTERIAL DISEASE BY THE DETOXIFICATION OF A PATHOGENIC TOXIN [J].

ANZAI, H ;

YONEYAMA, K ;

YAMAGUCHI, I .

MOLECULAR & GENERAL GENETICS, 1989, 219 (03) :492-494

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] OPTIMAL CONDITIONS FOR ORNITHINE CARBAMYL TRANSFERASE DETERMINATION - SIMPLE MICROMETHOD WITHOUT DEPROTEINIZATION [J].

CERIOTTI, G .

CLINICA CHIMICA ACTA, 1973, 47 (01) :97-105

[4] AN IMPROVED PROCEDURE FOR THE ISOLATION OF INTACT CHLOROPLASTS OF HIGH PHOTOSYNTHETIC CAPACITY [J].

CEROVIC, ZG ;

PLESNICAR, M .

BIOCHEMICAL JOURNAL, 1984, 223 (02) :543-545

[5] PHASEOLOTOXIN - CHLOROSIS, ORNITHINE ACCUMULATION AND INHIBITION OF ORNITHINE CARBAMOYLTRANSFERASE IN DIFFERENT PLANTS [J].

FERGUSON, AR ;

JOHNSTON, JS .

PHYSIOLOGICAL PLANT PATHOLOGY, 1980, 16 (02) :269-275

[6]

HARTMAN CL, 1985, PHYSIOL MOL PLANT P, V28, P353

[7] A SIMPLE AND GENERAL-METHOD FOR TRANSFERRING GENES INTO PLANTS [J].

HORSCH, RB ;

FRY, JE ;

HOFFMANN, NL ;

EICHHOLTZ, D ;

ROGERS, SG ;

FRALEY, RT .

SCIENCE, 1985, 227 (4691) :1229-1231

[8]

Maniatis T., 1982, MOL CLONING

[9] INVOLVEMENT OF PHASEOLOTOXIN IN HALO BLIGHT OF BEANS - TRANSPORT AND CONVERSION TO FUNCTIONAL TOXIN [J].

MITCHELL, RE ;

BIELESKI, RL .

PLANT PHYSIOLOGY, 1977, 60 (05) :723-729

[10]

MITCHELL RE, 1984, ANNU REV PHYTOPATHOL, V22, P215

← 1 2 3 →