AXIAL-BEAM ABSORBENCY DETECTION FOR CAPILLARY ELECTROPHORESIS

被引：34

作者：

TAYLOR, JA ^{[1
]}

YEUNG, ES ^{[1
]}

机构：

[1] IOWA STATE UNIV SCI & TECHNOL,DEPT CHEM,AMES,IA 50011

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 550卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)88586-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel approach to absorbance detection has been demonstrated for capillary electrophoresis utilizing an incident light beam directed along the capillary axis. The resulting absorption path length is roughly equal to the width of the sample bands present. This represents a 60-fold improvement in analytical path length over conventional on-column absorbance detectors without any loss of separation efficiency. The method can be used with completely aqueous buffer systems or those containing organic modifiers, and is applicable to both fused-silica and PTFE capillaries.

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页码：831 / 837

页数：7

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