DIFFERENTIATION OF FUSARIUM-SAMBUCINUM FUCKEL SENSU-LATO AND RELATED SPECIES BY RAPD PCR

被引:33
作者
HERING, O
NIRENBERG, HI
机构
[1] Institute of Microbiology, Federal Biological Research Centre for Agriculture and Forestry, Berlin
关键词
DNA EXTRACTION; FUNGI IMPERFECTI; FUSARIUM SAMBUCINUM; FUSARIUM TORULOSUM; FUSARIUM VENENATUM; RAPD PCR; SPECIES DIFFERENTIATION;
D O I
10.1007/BF01103341
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
DNA polymorphisms generated by the random amplified polymorphic DNA polymerase chain reaction (RAPD PCR) were used to analyse 41 isolates investigated in the European Fusarium sambucinum Project (EFSP). Employing ten arbitrary (10-mer) oligonucleotides and simple repeat sequences (M13, (GACA)(4)) as single primers, informative banding patterns typical for identifying European populations of Fusarium sambucinum Fuckel s. str., F. torulosum (Berk. and Curt.) Nirenberg and F. venenatum Nirenberg were obtained.
引用
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页码:159 / 164
页数:6
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