EFFECT OF ACTINOMYCIN-D ON TRYPANOSOMA-CRUZI

被引：6

作者：

DACRUZ, MQ

BRASCHER, HM

VARGENS, JR

OLIVEIRALIMA, A

机构：

[1] UNIV FED RIO DE JANEIRO, INST PUERICULTURA & PEDIAT, RIO DE JANEIRO, BRAZIL

[2] CTR PESQUISAS ARLINDO ASSIS FAP, BR-20031 RIO DE JANEIRO, BRAZIL

来源：

EXPERIENTIA | 1991年 / 47卷 / 01期

关键词：

ACTINOMYCIN-D; TRYPANOSOMA-CRUZI; VACCINATION;

D O I：

10.1007/BF02041263

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Viable metacyclic forms of T. cruzi, Y strain, treated with an adequate dose of actinomycin D (50-mu-g Act-D/ml/10(7) parasites/ml for 72 h at 28-degrees-C) showed the following properties: 1) they lost their ability to replicate in culture medium, in blood and in tissues of normal mice and were no longer able to incorporate tritiated thymidine; 2) they could not penetrate into Vero cells and could not replicate inside normal macrophages; 3) they retained their immunogenicity and the ability to protect mice against a virulent infection; 4) they did not induce histological lesions as described in chronic experimental Chagas' disease.

引用

页码：89 / 92

页数：4

共 9 条

[1] STUDIES OF MACROPHAGE COMPLEMENT RECEPTOR - ALTERATION OF RECEPTOR FUNCTION UPON MACROPHAGE ACTIVATION [J].