A STUDY OF CYTOCHROME-C-OXIDASE IN LIPID BILAYER - MEMBRANES ON ELECTRODE SURFACES

被引：87

作者：

CULLISON, JK

HAWKRIDGE, FM

NAKASHIMA, N

YOSHIKAWA, S

机构：

[1] VIRGINIA COMMONWEALTH UNIV,DEPT CHEM,RICHMOND,VA 23284

[2] NAGASAKI UNIV,DEPT APPL CHEM,NAGASAKI 852,JAPAN

[3] HIMEJI INST TECHNOL,FAC SCI,HIMEJI,HYOGO 67122,JAPAN

来源：

LANGMUIR | 1994年 / 10卷 / 03期

关键词：

D O I：

10.1021/la00015a044

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A membrane-associated enzyme, cytochrome c oxidase, has been put in lipid bilayer membranes attached to gold electrode surfaces. Gold/thiol self-assembly chemistry and deoxycholate dialysis procedures have been used to insert cytochrome c oxidase into a lipid bilayer on gold surfaces with a controlled orientation. Voltammetric and spectroelectrochemical results indicate that direct electron-transfer communication between the gold electrode surface and cytochrome c oxidase has been achieved. Moreover, immobilized cytochrome c oxidase can both reduce and oxidize solution-resident cytochrome c by appropriately controlling the applied electrode potential.

引用

页码：877 / 882

页数：6

共 68 条

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