MAINTENANCE OF METAPHASE IN COLCEMID-TREATED MOUSE EGGS BY DISTINCT CALCIUM-SENSITIVE AND 6-DIMETHYLAMINOPURINE (6-DMAP)-SENSITIVE MECHANISMS

In mouse eggs, the arrest at meiotic metaphase II is released by the fertilization-induced increase in intracellular calcium. When eggs treated with the microtubule inhibitor colcemid are fertilized or exposed to the calcium ionophore A23187, normal calcium increases occur, but the eggs remain at metaphase. However, when colcemid-treated eggs are fertilized or A23187-treated and then exposed to the protein kinase inhibitor 6-dimethylaminopurine (6-DMAP), they enter interphase. Although colcemid-treated eggs require a calcium signal and exposure to 6-DMAP, colcemid-treated embryonic cells are released from metaphase by treatment with 6-DMAP, but not by exposure to A23187. These results suggest that two distinct mechanisms maintain metaphase: one is the calcium-sensitive mechanism involving cytostatic factor, which normally maintains metaphase II arrest in eggs; the other mechanism, which may require the activity of 6-DMAP-sensitive kinases, maintains metaphase in the absence of spindle assembly in both mitotic cells and eggs. (C) 1995 Academic Press, Inc.