SEMIPREPARATIVE SEPARATION OF POLYHYDROXYLATED STEROLS USING A BETA-CYCLODEXTRIN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COLUMN

被引：13

作者：

WEST, RR ^{[1
]}

CARDELLINA, JH ^{[1
]}

机构：

[1] MONTANA STATE UNIV,DEPT CHEM,NAT PROD LAB,BOZEMAN,MT 59717

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 539卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(01)95356-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A series of closely related polyoxygenated sterols from the sponge Dysidea etheria was resolved by high-performance liquid chromatography on a beta-cyclodextrin column, a stationary phase apparently not used previously for the preparative separation of natural products. Analysis of the chromatographic behavior of the various sterols on the beta-cyclodextrin column indicated that formation of an inclusion complex was not the separation mechanism involved in this case. Rather, the comparative analysis suggested that hydrogen bonding between the solute molecules and the hydroxyl groups on the rim of the beta-cyclodextrin cavities best explained the results observed.

引用

页码：15 / 23

页数：9

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