DETERMINATION OF CHLORAMPHENICOL RESIDUES BY REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY IN FOIES GRAS

被引：5

作者：

CHEVALIER, M

POCHARD, MF

BEL, B

机构：

[1] Centre National d’Etudes Vètèrinaires et Alimentaires, Laboratoire Central d’Hygifène Alimentaire, Ministfère de l’Agriculture, Paris, 75015

来源：

FOOD ADDITIVES AND CONTAMINANTS | 1995年 / 12卷 / 01期

关键词：

CHLORAMPHENICOL; FOIES GRAS; LIQUID CHROMATOGRAPHY;

D O I：

10.1080/02652039509374284

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A reverse phase liquid chromatographic (LC) method for the determination of chloramphenicol (CAP) residues in foies gras was tested. The method was based on acetonitrile-sodium chloride extraction, sample clean-up with hexane and purification on a Sep-Pak C18 cartridge. Acceptable chromatographic separation was obtained for thawed samples with two sample clean-up steps with hexane, a purification step with acetonitrile + water (87.5 + 12.5) (v/v) before the elution of CAP residues by a mixture of acetonitrile + water (70 + 30) (v/v) with a chromatographic separation on Spherisorb ODS-2 (5 mu m) column using sodium acetate buffer (pH 4.3) + acetonitrile (78 +/- 22) (v/v) as mobile phase. The detection limit of the method was 2.5 mu g/kg. The mean recovery from spiked samples at the 10 mu g/kg level was 55.7 +/- 8.7%.

引用

页码：101 / 106

页数：6

共 6 条

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[2]

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[3]

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[4]

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[5]

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[6]

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