CONTROL OF AMMONIUM CONCENTRATION IN ESCHERICHIA-COLI FERMENTATIONS

被引：48

作者：

THOMPSON, BG ^{[1
]}

KOLE, M ^{[1
]}

GERSON, DF ^{[1
]}

机构：

[1] ALBERTA RES COUNCIL,DEPT BIOTECHNOL,11315 87TH AVE,EDMONTON T6G 2C2,ALBERTA,CANADA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1985年 / 27卷 / 06期

关键词：

AMMONIUM COMPOUNDS - BIOCHEMICAL ENGINEERING - CONTROL SYSTEMS - ELECTRODES; ELECTROCHEMICAL - Applications - HYDROGEN ION CONCENTRATION;

D O I：

10.1002/bit.260270610

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A control system has been devised for the maintenance of stable ammonium concentrations throughout a fed-batch fermentation. The control system is based on an ammonia gas-sensing electrode that monitors a pH-adjusted effluent stream from the fermentor. To overcome the time lag between the fermentor and the electrode, feedback control included metered flows of ammonium to both the fermentor and the electrode vessel. The system was used to study the growth of Escherichia coli B (ATCC 11303) at controlled ammonium concentrations of 5 to 200 mM.

引用

页码：818 / 824

页数：7

共 9 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] MUTANTS OF ESCHERICHIA-COLI REQUIRING METHIONINE OR VITAMIN-B12 [J].

DAVIS, BD ;

MINGIOLI, ES .

JOURNAL OF BACTERIOLOGY, 1950, 60 (01) :17-28

[3] COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].

DUBOIS, M ;

GILLES, KA ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356

[4]

HILL FF, 1982, PROCESS BIOCHEM, V17, P16

[5]

Kates M., 1972, TECHNIQUES LIPIDOLOG, V3, P268, DOI 10.1016/S0075-7535(08)70544-8

[6]

KIRCHNER JG, 1967, THIN LAYER CHROMATOG, V12

[7] GENETIC-CONTROL OF NITROGEN ASSIMILATION IN BACTERIA [J].

MAGASANIK, B .

ANNUAL REVIEW OF GENETICS, 1982, 16 :135-168

[8] REGULATION OF SYNTHESIS OF GLUTAMATE DEHYDROGENASE AND GLUTAMINE SYNTHETASE IN MICRO-ORGANISMS [J].

PATEMAN, JA .

BIOCHEMICAL JOURNAL, 1969, 115 (04) :769-&

[9] REGULATION OF NITROGEN-METABOLISM IN ESCHERICHIA-COLI AND KLEBSIELLA-AEROGENES - STUDIES WITH CONTINUOUS-CULTURE TECHNIQUE [J].

SENIOR, PJ .

JOURNAL OF BACTERIOLOGY, 1975, 123 (02) :407-418

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