SPECIFIC GROWTH-STIMULATION OF CULTURED SMOOTH-MUSCLE CELLS FROM SPONTANEOUSLY HYPERTENSIVE RATS BY PLATELET-DERIVED GROWTH-FACTOR A-CHAIN HOMODIMER

被引：33

作者：

RESINK, TJ ^{[1
]}

SCOTTBURDEN, T ^{[1
]}

HAHN, AWA ^{[1
]}

ROUGE, M ^{[1
]}

HOSANG, M ^{[1
]}

POWELL, JS ^{[1
]}

BUHLER, FR ^{[1
]}

机构：

[1] F HOFFMANN LA ROCHE & CO LTD, CH-4002 BASEL, SWITZERLAND

来源：

CELL REGULATION | 1990年 / 1卷 / 11期

关键词：

D O I：

10.1091/mbc.1.11.821

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cultured vascular smooth muscle cells (VSMC)1 from spontaneously hypertensive rats (SHR) possess specific cell surface receptors for both homodimeric forms of platelet-derived growth factor (PDGF-AA and PDGF-BB), in contrast to cells from normotensive Wistar Kyoto (WKY) animals, which express receptors only for the B-chain form of PDGF. Stimulation of quiescent VSMC from SHR with PDGF-AA resulted in activation of S6-kinase and induction of phosphoinositide catabolism, as well as cellular proliferation when cultures were maintained for prolonged periods with daily supplementation of the growth factor. WKY-derived VSMC showed no response to PDGF-AA, which was consistent with their lack of specific receptors for this homodimer. The responsiveness of quiescent cells from SHR and WKY to the B-chain homodimer was similar. The enhanced growth responsiveness of SHR-derived cells to fetal calf serum, as compared with cells from their normotensive counterparts, may be accounted for in part by their expression of receptors for the AA homodimer of PDGF. © 1990 by The American Society for Cell Biology.

引用

页码：821 / 831

页数：11

共 47 条

[1] TRANSFORMATION BY V-SIS OCCURS BY AN INTERNAL AUTOACTIVATION MECHANISM [J].

BEJCEK, BE ;

LI, DY ;

DEUEL, TF .

SCIENCE, 1989, 245 (4925) :1496-1499

[2]

BEVAN RD, 1976, BLOOD VESSELS, V13, P100

[3] RAPID DEVELOPMENT OF ATHEROSCLEROTIC LESIONS IN THE RABBIT CAROTID-ARTERY INDUCED BY PERIVASCULAR MANIPULATION [J].

BOOTH, RFG ;

MARTIN, JF ;

HONEY, AC ;

HASSALL, DG ;

BEESLEY, JE ;

MONCADA, S .

ATHEROSCLEROSIS, 1989, 76 (2-3) :257-268

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] SMOOTH-MUSCLE CELL IN CULTURE [J].

CHAMLEYCAMPBELL, J ;

CAMPBELL, GR ;

ROSS, R .

PHYSIOLOGICAL REVIEWS, 1979, 59 (01) :1-61

[6] POLYPEPTIDE GROWTH-FACTORS - ROLES IN NORMAL AND ABNORMAL-CELL GROWTH [J].

DEUEL, TF .

ANNUAL REVIEW OF CELL BIOLOGY, 1987, 3 :443-492

[7]

DICORLETO PE, 1988, GROWTH FACTOR PRODUC, V2, P51

[8]

FOLKOW B, 1973, CIRC RES, V32, P2

[9] MODIFIED LOW-DENSITY LIPOPROTEINS SUPPRESS PRODUCTION OF A PLATELET-DERIVED GROWTH FACTOR-LIKE PROTEIN BY CULTURED ENDOTHELIAL-CELLS [J].

FOX, PL ;

DICORLETO, PE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (13) :4774-4778

[10]

GRONWALD RGK, 1989, J BIOL CHEM, V264, P8120

← 1 2 3 4 5 →