A METHOD FOR THE DEMONSTRATION OF NADPH-DIAPHORASE ACTIVITY IN ANURAN SPECIES USING UNFIXED RETINAL WHOLEMOUNTS

被引:10
作者
GABRIEL, R
机构
[1] Department of Anatomy and Histology, The Flinders University of South Australia School of Medicine, Adelaide, SA
关键词
D O I
10.1679/aohc.54.207
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The presence of NADPH-diaphorase enzyme has been previously revealed in fixed mammalian retinal tissue (SAGAR, 1986). Fixed retinae of Bufo marinus and Xenopus laevis failed to yield selective staining when reacted for NADPH-diaphorase. Satisfactory staining of retinal neurons was attained when the histochemical reaction was carried out in unfixed retinal wholemounts. The applied method included the following steps: 1) Dissection of the fresh retina and the separation of the neural retina from all other coats of the eye ball, including the vitreal tissue; 2) pretreatment with 300 mM sucrose in phosphate buffer; 3) incubation with NADP, malic acid and nitroblue tetrazolium in phosphate buffer (pH 7.6); and 4) fixation of the tissue in 10% buffered formaldehyde overnight followed by whole mounting. For control, fixed and unfixed rabbit and human retinae were also reacted for NADPH-diaphorase according to the above method. In these species specific staining was achieved only with fixed tissues. The possible implications of revealing NADPH-diaphorase enzyme activity in fixed mammalian and non-fixed anuran tissues are discussed.
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页码:207 / 211
页数:5
相关论文
共 16 条
[1]   THE NUMBER OF NEURONS IN THE SMALL-INTESTINE OF MICE, GUINEA-PIGS AND SHEEP [J].
GABELLA, G .
NEUROSCIENCE, 1987, 22 (02) :737-752
[2]   ON THE NERVE PLEXUS OF THE CHICKEN GIZZARD [J].
GABELLA, G ;
HALASY, K .
ANATOMY AND EMBRYOLOGY, 1987, 177 (02) :97-103
[3]   TETRAZOLIUM STUDIES ON THE RETINA .3. ACTIVITY OF METABOLIC INTERMEDIATES AND MISCELLANEOUS SUBSTRATES [J].
KUWABARA, T ;
COGAN, DG .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1960, 8 (03) :214-224
[4]   DEVELOPMENT OF NADPH-DIAPHORASE CELLS IN THE RATS RETINA [J].
MITROFANIS, J .
NEUROSCIENCE LETTERS, 1989, 102 (2-3) :165-172
[5]   DISTRIBUTION OF REDUCED-NICOTINAMIDE-ADENINE-DINUCLEOTIDE-PHOSPHATE DIAPHORASE-POSITIVE CELLS AND FIBERS IN THE CAT CENTRAL NERVOUS-SYSTEM [J].
MIZUKAWA, K ;
VINCENT, SR ;
MCGEER, PL ;
MCGEER, EG .
JOURNAL OF COMPARATIVE NEUROLOGY, 1989, 279 (02) :281-311
[6]   NADPH-DIAPHORASE NEURONS OF HUMAN RETINAE HAVE A UNIFORM TOPOGRAPHICAL DISTRIBUTION [J].
PROVIS, JM ;
MITROFANIS, J .
VISUAL NEUROSCIENCE, 1990, 4 (06) :619-623
[7]   NADPH DIAPHORASE HISTOCHEMISTRY IN THE RABBIT RETINA [J].
SAGAR, SM .
BRAIN RESEARCH, 1986, 373 (1-2) :153-158
[8]   NADPH DIAPHORASE CELLS IN THE MAMMALIAN INNER RETINA [J].
SANDELL, JH .
JOURNAL OF COMPARATIVE NEUROLOGY, 1985, 238 (04) :466-472
[9]   HISTOCHEMICAL-DEMONSTRATION OF NADPH-DIAPHORASE ACTIVITY IN THE PINEAL ORGAN OF THE FROG (RANA-ESCULENTA), BUT NOT IN THE PINEAL ORGAN OF THE RAT [J].
SATO, T .
ARCHIVES OF HISTOLOGY AND CYTOLOGY, 1990, 53 (02) :141-146
[10]   NADPH-DIAPHORASE POSITIVE AMACRINE CELLS IN THE RETINAE OF THE FROG (RANA-ESCULENTA) AND PIGEON (COLUMBIA-LIVIA) [J].
SATO, T .
ARCHIVES OF HISTOLOGY AND CYTOLOGY, 1990, 53 (01) :63-69