SIMPLE METHOD FOR ISOLATION OF PRIMORDIAL GERM-CELL FROM CHICK-EMBRYOS

被引：25

作者：

CHANG, IK

TAJIMA, A

YASUDA, Y

CHIKAMUNE, T

OHNO, T

机构：

[1] UNIV TSUKUBA,INST AGR & FORESTRY,TSUKUBA,IBARAKI 305,JAPAN

[2] KUMAMOTO UNIV,SCH MED,DEPT ANAT,HONJO,KUMAMOTO 806,JAPAN

来源：

CELL BIOLOGY INTERNATIONAL REPORTS | 1992年 / 16卷 / 09期

关键词：

D O I：

10.1016/S0309-1651(06)80165-7

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A simple one step centrifugation method was developed for purification of primordial germ cells (PGCs) of chick embryos. PGCs, constituting less than 0.1% of the total blood cells of stage 13-14 embryos that contained a microliter amount of blood, were concentrated at the interface of a 6.3%(w/v) and 14.4%(w/v) Ficoll bilayer by centrifugation at 800 × g for 30 min. The purity of these PGCs was 86%, which was 22 times that obtained previously. © 1992 Academic Press Ltd.

引用

页码：853 / 857

页数：5

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