WHEAT AND BARLEY ALLERGENS ASSOCIATED WITH BAKERS ASTHMA - GLYCOSYLATED SUBUNITS OF THE ALPHA-AMYLASE-INHIBITOR FAMILY HAVE ENHANCED IGE-BINDING CAPACITY

被引：132

作者：

SANCHEZMONGE, R

GOMEZ, L

BARBER, D

LOPEZOTIN, C

ARMENTIA, A

SALCEDO, G

机构：

[1] ESCUELA TECN SUPER INGN AGRON,DEPT BIOQUIM,CIUDAD UNIV,E-28040 MADRID,SPAIN

[2] ALERGIA & INMUNOL ABELLO,E-28037 MADRID,SPAIN

[3] UNIV OVIEDO,FAC MED,DEPT BIOQUIM,E-33006 OVIEDO,SPAIN

[4] HOSP RIO HORTEGA,SERV ALERGIA,E-47010 VALLADOLID,SPAIN

来源：

BIOCHEMICAL JOURNAL | 1992年 / 281卷

关键词：

D O I：

10.1042/bj2810401

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 16 kDa protein, designated CM16*, which strongly binds IgE from baker's-asthma patients has been identified as a glycosylated form of the previously reported WTAI-CM16, which is a subunit of the wheat tetrameric alpha-amylase inhibitor. A glycosylated form (CMb*) of BTAI-CMb, the equivalent inhibitor subunit from barley, has been also found to have significantly enhanced IgE-binding capacity. In all, 14 purified members of the alpha-amylase/trypsin-inhibitor family showed very different IgE-binding capacities when tested by a dot-blot assay. The glycosylated components CM16* CMb* and the previously described non-glycosylated 14.5 kDa allergen from barley (renamed BMAI-1) were found to be the strongest allergens.

引用

页码：401 / 405

页数：5

共 25 条

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