DIFFERENTIAL G-PROTEIN MEDIATED COUPLING OF D2 DOPAMINE-RECEPTORS TO K+ AND CA2+ CURRENTS IN RAT ANTERIOR-PITUITARY-CELLS

In anterior pituitary cells, dopamine, acting on D2 dopamine receptors, concomitantly reduces calcium currents and increases potassium currents. These dopamine effects require the presence of intracellular GTP and are blocked by pretreatment of the cells with pertussis toxin, suggesting that one or more G protein is involved. To identify the G proteins involved in coupling D2 receptors to these currents, we performed patch-clamp recordings in the whole-cell configuration using pipettes containing affinity-purified polyclonal antibodies raised against either G(o-alpha), G(i3-alpha), or G(i1,2-alpha). Dialysis with G(o-alpha) antiserum significantly reduced the inhibition of calcium currents induced by dopamine, while increase of potassium currents was markedly attenuated only by G(i3-alpha) antiserum. We therefore conclude that in pituitary cells, two different G proteins are involved in the signal transduction mechanism that links D2 receptor activation to a specific modulation of the four types of ionic channels studied here.