APPLICATION OF A MONOCLONAL-ANTIBODY TO ESTIMATE RABBIT FIBRINOPEPTIDE-A RELEASED BY HABUTOBIN

被引：6

作者：

KINJOH, K ^{[1
]}

NAKAMURA, M ^{[1
]}

KOSUGI, T ^{[1
]}

机构：

[1] UNIV RYUKYUS,SCH MED,COMPREHENS MED RES CTR,NISHIHARA,OKINAWA 90301,JAPAN

来源：

TOXICON | 1994年 / 32卷 / 11期

关键词：

D O I：

10.1016/0041-0101(94)90413-8

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We reported previously that habutobin, one of the type A thrombin-like enzymes, releases fibrinopeptide A alone from rabbit fibrinogen. To evaluate the effective action of habutobin in experiments using rabbit for the treatment of thrombosis, we attempted to develop an immunological method for measuring the fibrinopeptide A level in the circulating blood of rabbit. The purified rabbit fibrinopeptide A was coupled to keyhole limpet hemocyanin and BALB/c mice were immunized with the resultant fibrinopeptide A-hemocyanin conjugate. The spleen cells of an immunized mouse were fused with myeloma cells (P3-X63-Ag8-U1). As' a result, one hybridoma (a-F-7) was selected, which secreted an antibody against rabbit fibrinopeptide A. Using this monoclonal antibody, we developed a competitive enzyme-linked immunoassay for estimating rabbit fibrinopeptide A. It was able to measure rabbit fibrinopeptide A contained in bentonite defibrinated plasma. This competitive enzyme-linked immunoassay should be useful for determining the fibrinopeptide A level in the circulating blood of rabbits, using plasma defibrinated by bentonite.

引用

页码：1413 / 1423

页数：11

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