IMPROVED PCR DETECTION OF CHLAMYDIA-TRACHOMATIS BY USING AN ALTERED METHOD OF SPECIMEN TRANSPORT AND HIGH-QUALITY ENDOCERVICAL SPECIMENS

被引：37

作者：

KELLOGG, JA ^{[1
]}

SEIPLE, JW ^{[1
]}

KLINEDINST, JL ^{[1
]}

STROLL, ES ^{[1
]}

CAVANAUGH, SH ^{[1
]}

机构：

[1] YORK HOSP,DEPT RES,YORK,PA 17405

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 10期

关键词：

D O I：

10.1128/JCM.33.10.2765-2767.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Duplicate endocervical swabs were collected for detection of Chlamydia trachomatis by PCR (Roche Diagnostics). One swab was swirled in Specimen Transport Medium (Roche) for PCR testing and discarded. A saline aliquot from the other specimen, sent as a dry swab to the laboratory, was Papanicolaou stained to determine specimen adequacy, and the remainder was PCR tested. Significantly more (24%) PCR-positive results (118 versus 95; P < 0.001) were obtained,vith the dry specimens than with the swirled specimens when first tested. In addition, PCR-positive results were obtained with 107 (10.6%) of 1,007 microscopically adequate specimens but with only 3 (0.9%) of 341 inadequate specimens (P < 0.001).

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页码：2765 / 2767

页数：3

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