STUDIES ON THE SEPARATION OF INTRACELLULAR SOLUBLE ENZYMES FROM BACTERIAL-CELL DEBRIS BY TANGENTIAL FLOW MEMBRANE FILTRATION

被引：19

作者：

DATAR, R

机构：

来源：

BIOTECHNOLOGY LETTERS | 1985年 / 7卷 / 07期

关键词：

D O I：

10.1007/BF01199861

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The recoveries of genetically engineered human growth hormone (hGH), carboxypeptidase and .beta.-galactosidase, from Escherichia coli broths were studied, using the process of microfiltration. The suspension superficial velocity was found to significantly influence the yields obtained. The MW of the product could be an important factor in microfiltration. Simple washing procedures seem to be adequate for effective rejuvenation of the membrane.

引用

页码：471 / 476

页数：6

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