MEMBRANE ENZYME REACTOR WITH SIMULTANEOUS SEPARATION USING ELECTROPHORESIS

被引：5

作者：

FURUSAKI, S

NOZAWA, T

NOMURA, S

机构：

[1] Department of Chemical Engineering, University of Tokyo, Tokyo

[2] Department of Agricultural Chemistry, University of Tokyo, Tokyo

来源：

BIOPROCESS ENGINEERING | 1990年 / 5卷 / 02期

关键词：

D O I：

10.1007/BF00589148

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A membrane enzyme reactor with simultaneous separation was investigated. Enzymes, urease and aspartase, were immobilized by a porous polytetrafluoroethylene membrane. Electrical field was applied in the medium while the reaction was carried out. Products with electrical charge could be separated through the membrane from the reaction medium as they were formed. Reaction behavior was analyzed by a simple model considering both pore-migration and reaction in the skelton of the membrane. According to the analysis the inherent reaction rate of the immobilized enzymes decreases significantly. This is probably caused by the structural variation of enzymes. For the case of urease, the change of pH inside the membrane may also cause the decrease of the reaction rate. The model analysis showed that the enzyme content in the membrane and the residence time of the substrate in the membrane governed overall extent of reaction. © 1990 Springer-Verlag.

引用

页码：73 / 78

页数：6

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