MORPHOGENETIC AND HORMONAL COMPONENTS OF THE HAIRY ROOT PHENOTYPE IN NICOTIANA-TABACUM CV XANTHI

被引:7
作者
CHRIQUI, D
TEPFER, M
PRINSEN, E
VANONCKELEN, H
机构
[1] Université P. et M. Curie, Laboratoire C.E.M.V, Paris Cédex 05, F-75252
[2] C.N.R.A, Laboratoire de Biologie cellulaire, Versailles
[3] U.S., A., Department of Biology, Wilrijk
来源
BULLETIN DE LA SOCIETE BOTANIQUE DE FRANCE-ACTUALITES BOTANIQUES | 1990年 / 137卷 / 3-4期
关键词
AUXINE; AGROBACTERIUM-RHIZOGENES; NICOTIANA-TABACUM; PHENOTYPE; HAIRY ROOT;
D O I
10.1080/01811789.1990.10827031
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Tobacco plants (Nicotiana tabacum cv. Xanthi) transformed by the left T-region (TL) of the agropine type strain A4 of Agrobacterium rhizogenes display modifications of the normal developmental pathways. These changes have been analysed regarding morphogenetic features and hormonal content. Transformed root clones submitted to bud regeneration give rise to transformed regenerants (T) displaying various phenotype abnormalities referred to as hairy-root syndrome, i.e., wrinkled leaves, shortened internodes, decreased apical dominance, longistyly, reduced seed production and highly branched root system, such traits being exaggerated in some plants (T'). DNA content of both transformed root apical meristems and T and T' shoot apices remains diploid, as revealed by DNA flow cytometry, indicating that phenotype alterations are not due to somaclonal variation through the regenerating process. Possible explanations for the phenotype of T and T' plants have been looked for by examining the functional parameters of the shoot apical meristems and the patterns of differentiation in the stems and leaves. Compared to N apices, which have only two loci for leaf initiation, T and T' meristems are generally enlarged, developing sometimes a third foliar helice. T and T' plants differ also from N by their degrees of meristematic activity (mitotic index, relative duration of the various phases of the cell cycle, nucleolar behaviour). Subapical cells of T and, moreover, T' plants display a delay of differentiation, a strong inhibition of longitudinal elongation and a stimulation of radial growth that can explain the shorter and larger internodes. In the T leaf primordia, a stimulation of mitotic activity indicates a delay in mesophyll differentiation while the vein tissues differentiate earlier leading to a precocious cessation of leaf vein elongation and to the wrinkled appearance. These morphogenetic deviations suggesting phytohormone imbalances, indoleacetic acid contents have been determined in apices, developing leaves and stems from N, T and T' plants. A possible clue to the hairy-root phenotype may be found in the low endogenous auxin levels in the transformed apices. These results will be discussed regarding the functional aspects of the TL genes and the correlations between hormonal levels and the corresponding phenotypes.
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页码:79 / 91
页数:13
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