MOLECULAR-BASIS FOR HETEROSIS FOR MYELIN BASIC-PROTEIN CONTENT IN MICE

被引：13

作者：

MISKIMINS, R ^{[1
]}

EBATO, H ^{[1
]}

SEYFRIED, TN ^{[1
]}

YU, RK ^{[1
]}

机构：

[1] YALE UNIV, DEPT NEUROL, NEW HAVEN, CT 06510 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1986年 / 83卷 / 05期

关键词：

D O I：

10.1073/pnas.83.5.1532

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Poly(A)+mRNA was isolated from the brains of C57BL/6J (B6), DBA/2J (D2), and F1 hybrid mice (B6 .times. D2) of 16-17 days of age. The yield of polysomal RNA, both poly(A)+ and poly(A)-, from the three strains of mice was comparable. When translated in vitro in a reticulocyte lysate system, the mRNA preparations had the same efficiency with respect to stimulation of amino acid incorporation into protein. However, a significant heterotic effect was seen for the production of myelin basic protein (MBP) by the mRNA from the F1 mice. That is, the fraction of protein synthesized as MBP was greater for the F1 hybrid than for either parental strain. The distribution of the form of MBPs was not different among the three strains. We therefore believe that heterosis for brain MBP content in the F1 hybrid may be regulated at the transcriptional or post-transcriptional level.

引用

页码：1532 / 1535

页数：4

共 13 条

[1] MYELIN BASIC-PROTEIN GENE-EXPRESSION IN QUAKING, JIMPY, AND MYELIN SYNTHESIS-DEFICIENT MICE [J].