A DNA PROBE WHICH DISTINGUISHES GLOBODERA-PALLIDA PA2/3 FROM G-ROSTOCHIENSIS RO1 AND OTHER CYST-FORMING NEMATODES

被引：2

作者：

MARSHALL, JW

CRAWFORD, AM

机构：

[1] Crop and Food Research Private Bag, Christchurch

[2] AgResearch Molecular Biology Unit, Biochemistry Department, University of Otago, Dunedin

来源：

NEW ZEALAND JOURNAL OF ZOOLOGY | 1992年 / 19卷 / 3-4期

关键词：

DNA PROBES; GLOBODERA-PALLIDA; G-ROSTOCHIENSIS; SPECIFICITY; SENSITIVITY;

D O I：

10.1080/03014223.1992.10422317

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

A small DNA fragment (approx. 350 base pairs) from the genome of the potato cyst nematode Globodera pallida Pa2/3 was cloned in a bacterial plasmid. When used as a probe in dot-blot DNA hybridisations against a range of nematodes, the cloned DNA bound to G. pallida Pa2/3 but not to Globodera rostochiensis Ro1. The cereal cyst nematode Heterodera avenae, the clover cyst nematode Heterodera trifolii, the root knot nematodes Meloidogyne hapla and Meloidogyne incognita, and the beet cyst nematode Heterodera schactii did not cross-hybridise. This probe can detect as few as six larvae of G. pallida.

引用

页码：133 / 138

页数：6

共 15 条

[1]

Birnboim H.C., A rapid alkaline extraction method for the isolation of Plasmid A, Methods in Enzymology, 100, pp. 243-255, (1983)

[2]

Burrows P.R., Perry R.N., Two cloned fragments which differentiate Globodera pallida from G. Rostochiensis, Revue De Nematology, 11, pp. 441-445, (1988)

[3]

Canto Saenz M., De Scurrah M.M., Races of the potato cyst nematode in the Andean region and a new system of classification, Nematologica, 23, pp. 340-349, (1978)

[4]

Curran J., McClure M.A., Webster J.M., Genotypic differentiation of Meloidogyne populations by detection of restriction fragment length difference in total DNA, Journal of Nematology, 18, pp. 83-86, (1986)

[5]

Fleming C.C., Marks R.J., The identification of potato cyst nematodes Globodera rostochiensis and G. Pallida by isoelectric focusing of proteins on polyacrylamide gels, Annals of Applied Biology, 103, pp. 277-281, (1983)

[6]

Fox P.C., Atkinson H.J., Glucose phosphate isomerase polymorphism in field populations of the potato cyst nematodes, Globodera rostochiensis and G. Pallida, Annals of Applied Biology, 104, pp. 503-506, (1984)

[7]

Franco J., Disc electrophoresis of female proteins of British and Peruvian potato cyst nematode populations Globodera spp, Nematologica, 25, (1979)

[8]

Kort J., Ross H., Rumpenhorst H.J., Stone A.R., An international scheme for identifying and classifying pathotypes of potato cyst nematodes Globodera rostochiensis and G. Pallida, Nematologica, 23, pp. 333-339, (1978)

[9]

Maniatis T., Fritsch E.F., Sambrook J., Molecular Cloning: A Laboratory Manual, (1982)

[10]

Marshall J.W., The Comparative Biology Ol Globodera Rostochiensis (Woll.) and Globodera Pallida (Stone) (Nematoda: Heteroderidae) in the South Island of New Zealand, (1984)

← 1 2 →