HPLC DETERMINATION OF ANTIOXIDANT SYNERGISTS AND ASCORBIC-ACID IN SOME FATTY PHARMACEUTICALS, COSMETICS AND FOOD

被引:16
作者
IRACHE, JM [1 ]
EZPELETA, I [1 ]
VEGA, FA [1 ]
机构
[1] UNIV NAVARRA,DEPT FARM & TECNOL FARMACEUT,E-31080 PAMPLONA,SPAIN
关键词
COLUMN LIQUID CHROMATOGRAPHY; ASCORBIC ACID; ANTIOXIDANT SYNERGISTS; FOOD; PHARMACEUTICALS AND COSMETICS;
D O I
10.1007/BF02269708
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A specific and sensitive reverse-phase HPLC method for the quantitative determination of ascorbic acid and antioxidant synergists (1-tartaric acid, citric acid, lactic acid as lithium lactate and EDTA) in fatty pharmaceuticals, cosmetics and food has been developed. Two extraction procedures were used; treatment with hot water, and extraction with water from a hexane dilution of the product. No significant differences between the two procedures were found (p < 0.05), except for ascorbic acid. Quantitative determinations were performed using a C-18 column and sulfuric acid (pH 1.95) mobile phase. With detection, at 210 nm, lactic acid overlapped with ascorbic acid, but the former could be readily identified by TLC. Ascorbic acid was detected at 254 nm, when lactic acid (as lithium lactate) did not interfere in the analysis. Mean recoveries for tartaric, citric and lactic acids were in the range 96-101 %.
引用
收藏
页码:232 / 236
页数:5
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