ENUMERATION OF ANTIGEN SITES ON CELLS BY FLOW-CYTOMETRY

被引：31

作者：

DAWSON, CD ^{[1
]}

SCHEPPLER, JA ^{[1
]}

NICHOLSON, JKA ^{[1
]}

HOLMAN, RC ^{[1
]}

机构：

[1] CTR DIS CONTROL,CTR INFECT DIS,DIV HOST FACTORS,STAT & DATA MANAGEMENT ACTIV,ATLANTA,GA 30333

来源：

PATHOBIOLOGY | 1991年 / 59卷 / 02期

关键词：

MONOCLONAL ANTIBODIES; LYMPHOCYTES; ANTIGEN DENSITY; MICROBEADS;

D O I：

10.1159/000163617

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We evaluated a cytofluorometric method for determining the number of antigens expressed on the cell surface of human lymphocytes. Using beads that have a known number of binding sites for mouse immunoglobulin and monoclonal antibodies specific for various antigens on human lymphocytes, we found that this system is quite reproducible, reliable and technically easy to perform. The greatest source of variation in expression of cell surface antigens is interdonor variability.

引用

页码：57 / 61

页数：5

共 14 条

[1] ANICHINI A, 1988, J IMMUNOL, V140, P183
[2] MODULATION OF MONOCYTE MO2 SURFACE-ANTIGEN EXPRESSION BY EXPOSURE TO RESPIRATORY SYNCYTIAL VIRUS
BAUMANN, MA
PAUL, CC
STOKES, C
BERNSTEIN, JM
[J]. AMERICAN JOURNAL OF HEMATOLOGY, 1987, 25 (03) : 337 - 340
[3] BOYUM A, 1968, SCAND J CLIN LAB INV, VS 21, P77
[4] HUMAN IMMUNODEFICIENCY VIRUS-INFECTION OF MONOCYTIC AND T-LYMPHOCYTIC CELLS - RECEPTOR MODULATION AND DIFFERENTIATION INDUCED BY PHORBOL ESTER
CLAPHAM, PR
WEISS, RA
DALGLEISH, AG
EXLEY, M
WHITBY, D
HOGG, N
[J]. VIROLOGY, 1987, 158 (01) : 44 - 51
[5] Cochran W.G, 1957, STAT METHODS, V6th ed
[6] Estabrook A, 1983, Diagn Immunol, V1, P199
[7] QUANTIFICATION BY FLOW CYTOFLUORIMETRY OF HLA CLASS-I MOLECULES AT THE SURFACE OF MURINE CELLS TRANSFORMED BY CLONED HLA GENES
LEBOUTEILLER, PP
MISHAL, Z
LEMONNIER, FA
KOURILSKY, FM
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 61 (03) : 301 - 315
[8] T-CELL SUBSETS DEFINED BY EXPRESSION OF LYT-1,2,3 AND THY-1 ANTIGENS - 2-PARAMETER IMMUNOFLUORESCENCE AND CYTO-TOXICITY ANALYSIS WITH MONOCLONAL-ANTIBODIES MODIFIES CURRENT VIEWS
LEDBETTER, JA
ROUSE, RV
MICKLEM, HS
HERZENBERG, LA
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1980, 152 (02) : 280 - 295
[9] LEHMANN EL, 1975, NONPARAMETRICS
[10] MASON DW, 1980, BIOCHEM J, V187, P1

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