NATIVE-TYPE DHP-SENSITIVE CALCIUM-CHANNEL CURRENTS ARE PRODUCED BY CLONED RAT AORTIC SMOOTH-MUSCLE AND CARDIAC ALPHA-1 SUBUNITS EXPRESSED IN XENOPUS-LAEVIS OOCYTES AND ARE REGULATED BY ALPHA-2-SUBUNIT AND BETA-SUBUNIT

被引：36

作者：

ITAGAKI, K

KOCH, WJ

BODI, I

KLOCKNER, U

SLISH, DF

SCHWARTZ, A

机构：

[1] UNIV CINCINNATI,COLL MED,DEPT PHARMACOL & CELL BIOPHYS,231 BETHESDA AVE,CINCINNATI,OH 45267

[2] DUKE UNIV,MED CTR,HOWARD HUGHES MED INST,DURHAM,NC 27710

来源：

FEBS LETTERS | 1992年 / 297卷 / 03期

关键词：

VOLTAGE-DEPENDENT CALCIUM CHANNEL; TISSUE-SPECIFIC SUBUNIT; COEXPRESSION; XENOPUS-LAEVIS OOCYTE;

D O I：

10.1016/0014-5793(92)80542-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Native tissue-like L-type voltage-dependent calcium channels (L-VDCC's) were expressed by in vitro transcribed cRNA injection of rat aorta or rabbit cardiac alpha(1) subunit into Xenopus laevis oocytes. Co-injection of VSM-alpha(1) with the cloned skeletal muscle beta-subunit (SK-beta) of the L-type VDCC significantly increased the expressed peak current amplitude without significant changes in kinetics. Similar results were obtained by co-injection of cardiac alpha(1) (DSHT-alpha(1)) the cloned skeletal alpha(2)-subunit (SK-alpha(2)) or with SK-beta. The oocytes co-expressing cRNA's retained L-type VDCC pharmacology.

引用

页码：221 / 225

页数：5

共 24 条

[1] CALCIUM CHANNELS IN MUSCLE-CELLS ISOLATED FROM RAT MESENTERIC-ARTERIES - MODULATION BY DIHYDROPYRIDINE DRUGS [J].