ZONA-FREE HAMSTER OVUM PENETRATION BY HUMAN-SPERMATOZOA - INFLUENCE OF VARIOUS SPERM FACTORS

被引：5

作者：

COURTOT, AM

JOUANNET, P

机构：

来源：

REPRODUCTION NUTRITION DEVELOPMENT | 1985年 / 25卷 / 03期

关键词：

D O I：

10.1051/rnd:19850404

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The influence of various technical factors when preparing human spermatozoa to be tested for their ability to penetrate zona-free hamster ova were evaluated. Higher in vitro fertilization rates were obtained after sperm selection by swim-up migration, 4-h incubation, sperm dilution to a concentration of 2.5 .times. 106 spermatozoa/ml, and observation of the oocytes 8 h after insemination. The use of control samples was necessary because of the wide intraindividual and interindividual variations in the results. Unless the result has been confirmed several times, it is impossible to conclude, from a negative test, that a human spermatozoon cannot penetrate a hamster oocyte.

引用

页码：521 / 529

页数：9

共 24 条

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WANG, YF
LIU, J
BEST, F
RICHARDSON, DW
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