CHARACTERIZATION OF A CDNA CLONE ENCODING THE COMPLETE AMINO-ACID-SEQUENCE OF COTTON ISOCITRATE LYASE

被引：41

作者：

TURLEY, RB ^{[1
]}

CHOE, SM ^{[1
]}

TRELEASE, RN ^{[1
]}

机构：

[1] ARIZONA STATE UNIV,DEPT BOT,TEMPE,AZ 85287

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1049卷 / 02期

关键词：

Amino acid sequence; cDNA; Cotton; Isocitrate lyase; Nucleotide sequence;

D O I：

10.1016/0167-4781(90)90045-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone encoding the glyoxysomal enzyme isocitrate lyase (ICL) (EC 4.1.3.1) was isolated from a library prepared from cotton (Gossypium hirsutum L.) cotyledon poly(A)+ RNA. The clone is 1893 basepairs (bp) in length and contains a 1728 bp open reading frame encoding a polypeptide of 576 residues (Mr = 64741). The deduced amino acid sequence of cotton ICL is 85.2%, 90.3% and 41.1% identical to ICL from rapeseed, castor bean and E. coli, respectively. Cotton ICL has a C-terminal tripeptide of A-R-M which is a putative trafficking signal for peroxisome (glyoxysome) proteins. © 1990.

引用

页码：223 / 226

页数：4

共 20 条

[1] NUCLEIC-ACID (CDNA) AND AMINO-ACID-SEQUENCES OF ISOCITRATE LYASE FROM CASTOR BEAN [J].