PHOSPHOLIPIDS ENHANCE THE BINDING OF PEPTIDES TO CLASS-II MAJOR HISTOCOMPATIBILITY MOLECULES

被引:68
作者
ROOF, RW
LUESCHER, IF
UNANUE, ER
机构
关键词
antigen presentation; antigen processing; antigen-presenting cells; immunogenicity; lipids;
D O I
10.1073/pnas.87.5.1735
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The binding of a lysozyme and ovalbumin peptide to purified class II major histocompatibility molecules in detergents was increased by the addition of certain lipids. Natural lipids from B lymphoma cells enhanced the binding and so did phosphatidylcholine, phosphatidylserine, phosphatidylinositol, and cardiolipin. Phosphatidylethanolamine, sphingomyelin, and cholesterol had no effect. There was no major difference between the effects of a phospholipid and its lys derivative. As studied with phosphatidylcholine, the increase in peptide binding was also dependent on the fatty acid composition of the lipid. The binding affinity was increased 10- to 50-fold in the presence of lipid as a result of an increase in the association rate while the off-rate remained essentially unchanged. Our results suggest that lipids, directly or indirectly, induce conformational changes in class II molecules that favor their peptide binding property.
引用
收藏
页码:1735 / 1739
页数:5
相关论文
共 39 条
[1]  
Allen P M, 1989, Int Immunol, V1, P141, DOI 10.1093/intimm/1.2.141
[2]   BINDING OF IMMUNOGENIC PEPTIDES TO IA HISTOCOMPATIBILITY MOLECULES [J].
BABBITT, BP ;
ALLEN, PM ;
MATSUEDA, G ;
HABER, E ;
UNANUE, ER .
NATURE, 1985, 317 (6035) :359-361
[3]   PENETRATION OF THE SIGNAL SEQUENCE OF ESCHERICHIA-COLI PHOE PROTEIN INTO PHOSPHOLIPID MODEL MEMBRANES LEADS TO LIPID-SPECIFIC CHANGES IN SIGNAL PEPTIDE STRUCTURE AND ALTERATIONS OF LIPID ORGANIZATION [J].
BATENBURG, AM ;
DEMEL, RA ;
VERKLEIJ, AJ ;
DEKRUIJFF, B .
BIOCHEMISTRY, 1988, 27 (15) :5678-5685
[4]   THE FOREIGN ANTIGEN-BINDING SITE AND T-CELL RECOGNITION REGIONS OF CLASS-I HISTOCOMPATIBILITY ANTIGENS [J].
BJORKMAN, PJ ;
SAPER, MA ;
SAMRAOUI, B ;
BENNETT, WS ;
STROMINGER, JL ;
WILEY, DC .
NATURE, 1987, 329 (6139) :512-518
[5]   A HYPOTHETICAL MODEL OF THE FOREIGN ANTIGEN-BINDING SITE OF CLASS-II HISTOCOMPATIBILITY MOLECULES [J].
BROWN, JH ;
JARDETZKY, T ;
SAPER, MA ;
SAMRAOUI, B ;
BJORKMAN, PJ ;
WILEY, DC .
NATURE, 1988, 332 (6167) :845-850
[6]   THE RELATION BETWEEN MAJOR HISTOCOMPATIBILITY COMPLEX (MHC) RESTRICTION AND THE CAPACITY OF IA TO BIND IMMUNOGENIC PEPTIDES [J].
BUUS, S ;
SETTE, A ;
COLON, SM ;
MILES, C ;
GREY, HM .
SCIENCE, 1987, 235 (4794) :1353-1358
[7]   INTERACTION BETWEEN A PROCESSED OVALBUMIN PEPTIDE AND IA MOLECULES [J].
BUUS, S ;
COLON, S ;
SMITH, C ;
FREED, JH ;
MILES, C ;
GREY, HM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (11) :3968-3971
[8]   ISOLATION AND CHARACTERIZATION OF ANTIGEN-IA COMPLEXES INVOLVED IN T-CELL RECOGNITION [J].
BUUS, S ;
SETTE, A ;
COLON, SM ;
JENIS, DM ;
GREY, HM .
CELL, 1986, 47 (06) :1071-1077
[9]   BINDING OF LABELED INFLUENZA MATRIX PEPTIDE TO HLA DR IN LIVING B-LYMPHOID CELLS [J].
CEPPELLINI, R ;
FRUMENTO, G ;
FERRARA, GB ;
TOSI, R ;
CHERSI, A ;
PERNIS, B .
NATURE, 1989, 339 (6223) :392-394
[10]   SPIN-LABEL ELECTRON-SPIN-RESONANCE STUDY OF BACTERIOPHAGE-M13 COAT PROTEIN INCORPORATION INTO MIXED LIPID BILAYERS [J].
DATEMA, KP ;
WOLFS, CJAM ;
MARSH, D ;
WATTS, A ;
HEMMINGA, MA .
BIOCHEMISTRY, 1987, 26 (24) :7571-7574