PRODUCTION OF RECOMBINANT XENOPUS FIBROBLAST GROWTH-FACTOR RECEPTOR-1 USING A BACULOVIRUS EXPRESSION SYSTEM

被引：6

作者：

BROWN, SAN ^{[1
]}

FRIESEL, R ^{[1
]}

机构：

[1] AMER RED CROSS,DEPT MOLEC BIOL,HOLLAND LAB,15601 CRABBS BRANCH WAY,ROCKVILLE,MD 20855

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 193卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1741

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A baculovirus expression system has been used to express large quantities of full-length biologically active Xenopus fibroblast growth factor receptor-1. Recombinant XFGFR-1 was purified to near homogeneity by a single step immunoaffinity purification procedure. The recombinant XFGFR-1 binds both FGF-1 and FGF-2 with high affinity. XFGFR-1 undergoes autophosphorylation principally on tyrosine residues in an immuno-complex kinase assay. Thus, recombinant XFGFR-1 expressed in insect cells is biologically active and suitable for structural and functional analysis. © 1993 Academic Press, Inc.

引用

页码：1116 / 1122

页数：7

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