Pericardial patch venoplasty heals via attraction of venous progenitor cells

被引：24

作者：

Bai, Hualong ^{[1
,2
,3
,4
]}

Wang, Mo ^{[1
,2
,5
]}

Foster, Trenton R. ^{[1
,2
]}

Hu, Haidi ^{[1
,2
,6
]}

He, Hao ^{[1
,2
,5
]}

Hashimoto, Takuya ^{[1
,2
,6
]}

Hanisch, Jesse J. ^{[1
,2
]}

Santana, Jeans M. ^{[1
,2
]}

Xing, Ying ^{[3
]}

Dardik, Alan ^{[1
,2
,6
]}

机构：

[1] Yale Univ, Sch Med, Vasc Biol & Therapeut Program, New Haven, CT USA

[2] Yale Univ, Sch Med, Dept Surg, New Haven, CT 06510 USA

[3] Zhengzhou Univ, Basic Med Coll, Zhengzhou, Henan, Peoples R China

[4] Zhengzhou Univ, Affiliated Hosp 1, Dept Vasc Surg, Zhengzhou, Henan, Peoples R China

[5] Cent South Univ, Xiangya Hosp 2, Dept Vasc Surg, Changsha, Hunan, Peoples R China

[6] VA Connecticut Healthcare Syst, Dept Surg, West Haven, CT USA

来源：

PHYSIOLOGICAL REPORTS | 2016年 / 4卷 / 12期

基金：

美国国家卫生研究院;

关键词：

Aortocaval fistula; arterioplasty; arteriovenous fistula; AVF ligation; Eph-B4; pericardial patches; pseudoaneurysm; venoplasty; venous neointimal hyperplasia;

D O I：

10.14814/phy2.12841

中图分类号：

Q4 [生理学];

学科分类号：

071003 [生理学];

摘要：

Pericardial patches are commonly used during cardiovascular surgery to close blood vessels. In arteries, patches accumulate arterial progenitor cells; we hypothesized that venous patches would accumulate venous progenitor cells, in the absence of arterial pressure. We developed a novel rat inferior vena cava (IVC) venotomy model and repaired it with a pericardial patch. Cells infiltrated the patch to form a thick neointima by day 7; some cells were CD34(+)/VEGFR2(+) and CD31(+)/Eph-B4(+) consistent with development of venous identity in the healing patch. Compared to arterial patches, the venous patches had increased neointimal thickness at day 7 without any pseudoaneurysms. Addition of an arteriovenous fistula (AVF) to increase blood flow on the patch resulted in reduced patch neointimal thickness and proliferation, but neointimal thickness was not reversible with AVF ligation. These results show that rat patch venoplasty is a novel model of aggressive venous neointimal hyperplasia.

引用

页数：13

共 28 条

[1]

Delivery of mesenchymal stem cells in biomimetic engineered scaffolds promotes healing of diabetic ulcers [J].