RECOMMENDED PROTOCOLS BASED ON A SURVEY OF CURRENT PRACTICE IN GENOTOXICITY TESTING LABORATORIES .4. CHROMOSOME ABERRATION AND SISTER-CHROMATID EXCHANGE IN CHINESE-HAMSTER OVARY, V79 CHINESE-HAMSTER LUNG AND HUMAN LYMPHOCYTE-CULTURES

被引：109

作者：

SWIERENGA, SHH

HEDDLE, JA

SIGAL, EA

GILMAN, JPW

BRILLINGER, RL

DOUGLAS, GR

NESTMANN, ER

机构：

[1] CANTOX INC, OAKVILLE L6J 5Z7, ONTARIO, CANADA

[2] UNIV GUELPH, GUELPH N1G 2W1, ONTARIO, CANADA

[3] YORK UNIV, DEPT BIOL, N YORK M3J 1P3, ONTARIO, CANADA

[4] HLTH & WELF CANADA, HLTH PROTECT BRANCH, ENVIRONM HLTH DIRECTORATE, OTTAWA K1A 0L2, ONTARIO, CANADA

来源：

MUTATION RESEARCH | 1991年 / 246卷 / 02期

关键词：

RECOMMENDED PROTOCOL; CURRENT USAGE; CHROMOSOME ABERRATION; SISTER-CHROMATID EXCHANGE; CHO; V79; HUMAN LYMPHOCYTES;

D O I：

10.1016/0027-5107(91)90050-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A recommended protocol has been developed for chromosomal aberration and sister-chromatid exchange assays in CHO, V79 and human lymphocyte cultures. The protocol was based on the responses to a detailed questionnaire completed by North-American and European governmental, university, and contract laboratories using these tests. This report identifies those modifications to previously described methods that are used on a regular basis and clarifies confusing or inconsistent practices. These protocols can be modified for use in other types of cells.

引用

页码：301 / 322

页数：22

共 39 条

[1]

ASHLEY J, 1989, ENVIRON MOL MUTAGEN, V14, P51

[2] MUTAGENESIS BY CHEMICAL-AGENTS IN V79 CHINESE-HAMSTER CELLS - A REVIEW AND ANALYSIS OF THE LITERATURE - A REPORT OF THE GENE-TOX PROGRAM [J].

BRADLEY, MO ;

BHUYAN, B ;

FRANCIS, MC ;

LANGENBACH, R ;

PETERSON, A ;

HUBERMAN, E .

MUTATION RESEARCH, 1981, 87 (02) :81-142

[3] GENOTOXIC EFFECTS IN CULTURED-MAMMALIAN-CELLS PRODUCED BY LOW PH TREATMENT CONDITIONS AND INCREASED ION CONCENTRATIONS [J].

BRUSICK, D .

ENVIRONMENTAL MUTAGENESIS, 1986, 8 (06) :879-886

[4]

BRUSICK D, 1987, PRINCIPLES GENETIC T, P193

[5]

Brusick D., 1989, PRINCIPLES METHODS T, VSecond, P407

[6]

Danford N., 1985, PROGR MUTATION RES, V5, P397

[7]

Dean B.J., 1984, MUTAGENICITY TESTING, P187

[8] HUMAN PERIPHERAL-BLOOD LYMPHOCYTES FOR ANALYSIS OF CHROMOSOME-ABERRATIONS IN MUTAGEN TESTS [J].

EVANS, HJ ;

ORIORDAN, ML .

MUTATION RESEARCH, 1975, 31 (03) :135-148

[9]

EVANS HJ, 1977, HDB MUTAGENICITY TES, P405

[10]

GAD SC, 1989, PRINCIPLES METHODS T, P435

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